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I-oyile ye-Dalbergia Odoriferae esulungekileyo ye-Lignum ye-oyile yekhandlela kunye nesepha eyenza i-hoseile idiffuser i-oyile ebalulekileyo entsha kwizitshisi zeengcongolo.

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Isityalo samayezaI-Dalbergia odoriferaT. Chen iintlobo, ezikwabizwa ngokubaLignum Dalbergia odoriferae[1], yeyeqelaeDalbergia, usapho lweFabaceae (Leguminosae) [2]. Esi sityalo sisasazwe ngokubanzi kwiindawo ezishushu zoMbindi noMzantsi Merika, eAfrika, eMadagascar, naseMpuma nakumaZantsi eAsia [1,3], ngakumbi eTshayina [4].D. odoriferaiintlobo, ekwaziwa ngokuba yi "Jiangxiang" ngesiTshayina, "Kangjinhyang" ngesiKorea, kunye ne "Koshinko" kumayeza aseJapan, isetyenziswe kumayeza esintu ukunyanga izifo zentliziyo, umhlaza, isifo seswekile, ukuphazamiseka kwegazi, ischemia, ukudumba. i-necrosis, iintlungu ze-rheumatic, njl.5-7]. Ngokukodwa, ukusuka kumalungiselelo e-herbal aseTshayina, i-heartwood yafunyanwa kwaye isetyenziswe ngokuqhelekileyo njengenxalenye yemixube yeziyobisi zorhwebo kunyango lwe-cardiovascular treatment, kubandakanywa i-decoction ye-Qi-Shen-Yi-Qi, iipilisi ze-Guanxin-Danshen, kunye ne-Danshen injection [5,6,8-11]. Njengabanye abaninzieDalbergiaiintlobo, uphando lwe-phytochemical lubonise ukwenzeka kwezinto eziphambili ze-flavonoid, phenol, kunye ne-sesquiterpene kwiindawo ezahlukeneyo zesi sityalo, ngakumbi malunga ne-heartwood [12]. Ngapha koko, inani leengxelo ze-bioactive malunga ne-cytotoxic, i-antibacterial, i-antioxidative, i-anti-inflammatory, i-antithrombotic, i-antiosteosarcoma, i-antiosteoporosis, kunye nemisebenzi ye-vasorelaxant kunye nemisebenzi ye-alpha-glucosidase inhibitory ibonisa ukuba zombiniD. odoriferaizicatshulwa ezikrwada kunye neemetabolites zayo zesibini zizixhobo ezixabisekileyo kuphuhliso lwamachiza amatsha. Nangona kunjalo, akukho bungqina buchazwe kwimbono jikelele malunga nesi sityalo. Kolu hlaziyo, sinika amagqabantshintshi amacandelo amakhulu ekhemikhali kunye novavanyo lwebhayoloji. Olu hlaziyo luya kwenza igalelo ekuqondeni amaxabiso emveliD. odoriferakunye nezinye iintlobo ezinxulumeneyo, kwaye ibonelela ngezikhokelo eziyimfuneko kuphando lwexesha elizayo.


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    Ngokutsho kwedathabheyisi yoLuhlu lweZityalo (http://www.theplantlist.org, 2017), igama elilandelayo elamkelekileyo leI-Dalbergia odoriferaT. Chen iintlobo zidweliswe kwinqanaba lokuzithemba okuphezulu [13]. Isityalo samayezaD. odoriferaiintlobo, ezikwaziwa njenge rosewood enevumba elimnandi, ngumthi onyakamayo onencindi [14], ezineempawu zemopholoji ezifana nobude obuziimitha ezingama-30–65, amagqabi ombhoxo, kunye neentyatyambo ezincinane ezityheli [14]. I-morphology yeempawu nayo iye yaxelwa kumsebenzi kaHao noWu (1993), ngokusekwe kwinkcazo eneenkcukacha yokwakheka komzimba kunye nesakhiwo sangaphandle esenziwe kwiiseli ze-parenchyma zesiqu somthi ovuthulukayo wetropikhi.D. odoriferaiintlobo [15]. Njengoko iziphumo zibonisiwe, kwi-phloem yesibini ye-branchlet kunye ne-trunk, iiprotheni ze-vacuole zifunyenwe kuzo zonke iiseli ze-parenchyma, ngaphandle kweeseli zeqabane. Ukongeza, iiprotheni ezikwi-ray parenchyma kunye ne-vasicentric parenchyma zivele kuphela kwi-xylem yesibini yangaphandle ye-branchlet, kodwa hayi kwi-trunk yesibini ye-xylem. Iiprotheni ze-xylem vacuole ziqokelelwe ekupheleni kwexesha lokukhula kwaye zanyamalala emva kokukhula kokuqala kokukhula entwasahlobo. Iiprotheni ze-phloem vacuole zibonise ukuhluka kwexesha, ngokukodwa kwiiseli ezikufutshane ne-cambium. Ubume be-fibrous yeeprotheyini ze-vacuole ngokucacileyo bufunyenwe kwimeko yokudibanisa okanye ngaphezulu okanye ngaphantsi kwe-dispersion eyenzekayo kwii-vacuoles ezinkulu ezisembindini ngexesha lokukhula kunye nexesha lokulala. Okubalulekileyo, indalo yophuhliso lwamaxesha onyaka kwimithi yetropikhi isenokwahluka kuleyo yemithi epholileyo, apho umthi wemidumba osuka kwiindawo ezishushu zase China njengeD. odoriferaIintlobo zineeproteni zokugcina isiqu kwii-vacuoles ezinkulu, kodwa iiproteni zokugcina iziqu zemithi epholileyo zavela njengeeprotein ezincinci zokugcina iiprotheyini okanye imizimba yeprotheyini, kunye nohlobo oluthile lokugcinwa kweprotheyini efunyenwe kwizityalo ezitshatyalaliswayo zisenokungabi yinto eyenzeka ngengozi [15].

    Isityalo samayezaD. odoriferaiintlobo ziye zaboniswa njengenye yemithi yerosewood exabisekileyo emhlabeni enamayeza ahlukeneyo kunye namaxabiso aphezulu orhwebo. Ngokomzekelo, i-heartwood yayo, ebizwa ngokuba yi "Jiangxiang" kumayeza emveli aseTshayina, yayisetyenziswa kwi-Pharmacopoeia yaseTshayina ukunyanga izifo zentliziyo, umhlaza, isifo seswekile, ukuphazamiseka kwegazi, ischemia, ukudumba, i-necrosis, kunye neentlungu ze-rheumatic.6,7]. Njengoko sisazi, i-heartwoods ibonelele ngomthombo onenzuzo weeoli eziyimfuneko, ezinokubonwa njengento exabisekileyo yokulungisa iziqholo [1]. Ngaphandle kwendima ebalulekileyo kwishishini lamayeza, i-heartwoods yayidume ngefanitshala yodidi oluphezulu kunye nobugcisa, ngenxa yevumba labo elimnandi, indawo entle, kunye nokuxinana okuphezulu [2]. Kuyaphawuleka ukuba isityalo sasendleD. odoriferaiintlobo zezilwanyana zisemngciphekweni wokulahleka kwendawo yokuhlala kunye nokuxhatshazwa ngokugqithisileyo ekusetyenzisweni kwamaplanga [2,16]. Ke ngoko, ukukhuselwa kunye nokukhula kwalo ngumsebenzi ongxamisekileyo. Ngokunxuseneyo noku, kutshanje, impembelelo yokwahluka kwejografi kunye nobushushu kwiD. odoriferaukuntshula kwembewu (ngokusekelwe kwiindawo ezine zejografi: iLedong, iHainan; iPingxiang, iGuangxi Zhuang Autonomous Region; iZhaoqing, iGuangdong; neLonghai, eFujian, eTshayina) kwanikelwa ingxelo ngayo kumsebenzi kaLiu et al. (2017) [16]. Isiphumo sidize ukuba elona qondo lobushushu lokuntshula kwembewu eqokelelwe eLedong nasePingxiang yayingama-25°C, kanti yona imbewu kwezimbini ziseleyo yayingama-30°C. Kwelinye icala, uLu et al. (2012) yafumanisa ukuba amandla okuqhuqha ukulungisa i-N2 ukusuka emoyeni ngaphakathiD. odoriferaIintlobo zaziyimfuneko ekumiselweni nasekukhuleni kwezithole, kwaye ke ngoko kufuneka sichonge ubudlelwane be-symbiosis phakathi kweentlobo ze-rhizobia kunye namaqhuqhuva.D. odoriferaiintlobo [17]. Uhlalutyo lwe-Phylogenetic lwe-16S rRNA gene kunye ne-16S-23S yangaphakathi ekhutshelweyo yesikhala (ITS) ithathele ingqalelo ukuba ezi ntlobo zimbini zebhaktiriya, i-8111 kunye ne-8201, zazibekwe zodwa kumaqhuqhuva eengcambu zembotyi ehlala ikho kuMazantsi eTshayina,D. odoriferaiintlobo, ezazinxulumene ngokusondeleyoBurkholderia cepacia. Okwangoku, zazifana nokusetyenziswa komthombo wekhabhoni usebenzisa i-biology yovavanyo lweplate ye-GN2 kunye nomxholo wabo we-DNA G + C wawuyi-65.8 kunye ne-65.5 mol%, ngokulandelanayo [17]. Iintlobo ezimbini zeentlobo, i-8111 kunye ne-8201, zibonelele ngokufana okuphezulu kunyeB. cepaciaentsonkothileyo kwi oxidation phantse yonke imithombo carbon, ngaphandle cellobiose, xa kuthelekiswaB. cepaciakwayeB. pyrrociniange-oxidation ye-cellobiose kunye ne-xylitol kunyeB. Vietnamesengokuxutywa kwe-adonitol kunye ne-cellobiose.17]. Ukongeza, i-biomass yesityalo kunye nomxholo we-N ubonise ukuba ukulungiswa okusebenzayo kwe-N2 kwenzeka kumaqhuqhuva emva kokugonywa kwezi zimbini.Burkholderiaiintlobo, xa kuthelekiswa nezithole zolawulo olubi lweD. odoriferaiintlobo [17]. Ukuququmbela,BurkholderiaIintlobo ze-8111 kunye ne-8201 zinokudlala indima efanelekileyo ekwenzeni amaqhuqhuva asebenzayo eentlobo zemidumbaD. odorifera[17].

    I-Endophytic fungi okanye i-endophytes, ekhoyo ngokubanzi ngaphakathi kwizihlunu ezisempilweni zezityalo, inokuphembelela kakhulu ukwenziwa kweemveliso zemetabolism kunye nomgangatho kunye nobungakanani beemveliso zendalo ezivela kwizityalo zamayeza [49]. Ubudlelwane phakathi kweentlobo ngeentlobo zefungi kunye nenxalenye yentliziyo engaqhelekanga yaseGuangdong, eTshayina,D. odoriferaiintlobo, kwabikwa ngu Sun et al. (2015); okokuqala, zimbini kuphela iifungi ezibekwe zodwa ukusuka 160 ezimhlophe iinkuni eziphilileyo iithishu, malunga neminyaka esixhenxe ubudala, ezidityaniswe iintlobo Bionectriaceae. Ngokuchasene noko, ii-85 fungi zachongwa kwizicubu zomthi ezimfusa okanye ezimfusa-mdaka, ezimalunga neminyaka esixhenxe ubudala, kwaye zaziyinxalenye ye-12 yeentlobo.2]. Okwesibini, ukuchongwa kweemolekyuli kunye nohlalutyo lwe-phylogenetic lubonise ukuba i-fungi yodwa yenza amacandelo asixhenxe ahlukeneyo kunye nobuninzi bexabiso le-bootstrap ngaphezu kwe-90%, kubandakanywa.I-Fusariumsp., Bionectriaceae, Pleosporales,Phomopsissp.,Exophiala jeanselmei,I-Auricularia polytricha, kwayeOudemansiellasp. Ngokomzekelo, ulandelelwano lwe-ITS oluvela kwikhowudi esecaleni 12120 ukusuka kumthi olimeleyo ichongiwe njengePhomopsissp. kwaye yadityaniswa yi-98% yenkxaso ye-bootstrap ngePhomopsissp.DQ780429okanye ngekhowudi esecaleni engu-12201 ephuma kumthi omhlophe osempilweni, usebenzisa iclade exhaswa ngamandlaI-Bionectriaceaesp.EF672316, ngakumbi ezintathu zodwa i-12119, 12130, kunye ne-12131 ezazisondelelene nexabiso le-92% ye-bootstrap, eyadityaniswa ngamandla kunye nolandelelwano lwereferensi yeI-Fusariumsp. kwiGenBank. Okwesithathu, uphando olubanzi kunye nocazululo olubanzi lwe-endophytic isolation frequency luveze iindidi ezilishumi elinambini zokungunda kwiplanga elenzakeleyo elimfusa-bumdaka apho ubukoloniyali bubonke bungama-53.125%, bodidi olusibhozo okanye iintsapho:Eutypa,I-Fusarium,Phomopsis,Oudemansiella,Eutypella,I-Auricularia,IiPleoporalessp., kwayeExophiala, apho kuEutypasp. (12123) yayiyeyona ixhaphakileyo nge-21.25%, kanti kuphelaI-Bionectriaceaesp. (1.25%) yafunyanwa kumthi omhlophe osempilweni. Ekugqibeleni, uhlalutyo lwe-anatomical lubonisa ukuba i-hyphae ethile yokungunda yavela kwiinqanawa zomthi owenziwe ngamanxeba amfusa-bumdaka, ngelixa yona ayizange ifumaneke kwinqanawa yomthi omhlophe ophilileyo.








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