Ioyile ye-Artemisia capillaris esulungekileyo yekhandlela kunye nesepha yenza i-oyile ethengisayo idiffuser ebalulekileyo entsha kwizitshisi zeengcongolo.

I-oyile ye-Artemisia capillaris esulungekileyo yekhandlela kunye nesepha yenza i-hoseile diffuser ioli eyimfuneko entsha yesitshisi sengcongolo isitshisi sengcongolo

inkcazelo emfutshane:

Uyilo lwemodeli yempuku

Izilwanyana zahlulwa ngokungenamkhethe zangamaqela amahlanu aneshumi elinesihlanu ngalinye. Iqela lolawulo kunye neempuku zeqela lemodeli zaye zagatywaioli yesesameiintsuku ezi-6. Iigundane zeqela elilungileyo zahluthwa kunye neepilisi ze-bifendate (BT, 10 mg / kg) kwiintsuku ze-6. Amaqela okulinga aphathwa nge-100 mg / kg kunye ne-50 mg / kg i-AEO echithwe kwioli yeesame kwiintsuku ze-6. Ngomhla we-6, iqela lokulawula liphathwe ngeoli yeesame, kwaye onke amanye amaqela aphathwa ngedosi enye ye-0.2% CCl4 kwioli yeesame (10 ml / kg) ngo.inaliti ye-intraperitoneal. Emva koko iigundane zazizila ngaphandle kwamanzi, kwaye iisampuli zegazi zaqokelelwa kwiinqanawa ze-retrobulbar; igazi eliqokelelweyo laliyi-centrifuged kwi-3000 ×gkuba 10 min ukwahlula serum.Ukukhutshwa komlomo wesibelekokwenziwa ngokukhawuleza emva kokurhoxiswa kwegazi, kwaye iisampulu zesibindi zasuswa ngokukhawuleza. Enye inxalenye yesampulu yesibindi yagcinwa ngokukhawuleza kwi-−20 °C de uhlalutyo, kwaye enye inxalenye yahlanjululwa kwaye yalungiswa kwi-10%formalinisisombululo; izihlunu eziseleyo zagcinwa ku-−80 °C ukuze kuhlalutywe i-histopathological (Wang et al., 2008,Hsu et al., 2009,Nie et al., 2015).

Umlinganiselo weparameters biochemicals kwiserum

Ukulimala kwesibindi kwavavanywa ngokuqikelelaimisebenzi enzymaticye-serum ALT kunye ne-AST usebenzisa iikiti zorhwebo ezihambelanayo ngokwemiyalelo yeekiti (Nanjing, kwiPhondo laseJiangsu, eChina). Imisebenzi ye-enzymatic yachazwa njengeeyunithi kwilitha nganye (U/l).

Umlinganiselo we-MDA, SOD, GSH kunye ne-GSH-Pxkwi-homogenates yesibindi

Izicubu zesibindi zenziwe nge-homogenized kunye ne-saline ye-physiological ebandayo kwi-1: umlinganiselo we-9 (w / v, isibindi: i-saline). I-homogenates yayiyi-centrifuged (2500 ×gkwimizuzu eyi-10) ukuqokelela amandla amakhulu ukuze kumiselwe okulandelayo. Ukulimala kwesibindi kwavavanywa ngokwemilinganiselo ye-hepatic ye-MDA kunye ne-GSH amanqanaba kunye ne-SOD kunye ne-GSH-Pximisebenzi. Zonke ezi zimiselwe ngokulandela imiyalelo ekwikhithi (eNanjing, kwiPhondo laseJiangsu, eTshayina). Iziphumo ze-MDA kunye ne-GSH zichazwe njenge-nmol ngeprotheyini ye-mg (nmol / mg prot), kunye nemisebenzi ye-SOD kunye ne-GSH-Pxzavezwa njenge-U per mg protein (U/mg prot).

Uhlalutyo lwe-Histopathological

Izahlulo zesibindi esandula ukufunyanwa zilungisiwe kwi-10% ye-bufferedparaformaldehydeisisombululo sephosphate. Isampulu emva koko ifakwe kwiparafini, yasikwa ibe ngamacandelo angama-3-5 μm, adyojwe nge.hematoxylinkwayeeosin(H&E) ngokwenkqubo eqhelekileyo, kwaye ekugqibeleni ihlalutywe nguimicroscope elula(UTian et al., ngo-2012).

Uhlalutyo lwamanani

Iziphumo zachazwa njengentsingiselo ± ukutenxa okusemgangathweni (SD). Iziphumo zahlalutywa kusetyenziswa inkqubo yamanani-nkcazo SPSS Statistics, version 19.0. Idatha yayiphantsi kohlalutyo lokungafani (ANOVA,p< 0.05) elandelwa luvavanyo lukaDunnett kunye novavanyo lweT3 lukaDunnett ukumisela umahluko obalulekileyo ngokweenkcukacha-manani phakathi kwexabiso lamaqela ahlukeneyo ovavanyo. Umahluko obalulekileyo waqwalaselwa kwinqanabap< 0.05.

Iziphumo neengxoxo

Amalungu e-AEO

Ekuhlalutyweni kweGC / MS, i-AEO ifunyenwe iqulethe i-25 ekhutshwe kwi-10 ukuya kwi-35 min, kwaye i-21 i-accounting ye-84% yeoli efunekayo ichongiwe.Uluhlu loku-1). Ioli eguquguqukayo equlethwemonoterpenoids(80.9%), i-sesquiterpenoids (9.5%), i-saturated unbranched hydrocarbons (4.86%) kunye ne-miscellaneous acetylene (4.86%). Xa kuthelekiswa nezinye izifundo (Guo et al., 2004), sifumene i-monoterpenoids eninzi (80.90%) kwi-AEO. Iziphumo zibonise ukuba eyona ndawo ininzi ye-AEO yi-β-citronellol (16.23%). Amanye amacandelo amakhulu e-AEO aquka i-1,8-cineole (13.9%),i-camphor(12.59%),linalool(11.33%), α-pinene (7.21%), β-pinene (3.99%),thymol(3.22%), kunyemyrcene(2.02%). Ukwahluka kokwakheka kweekhemikhali kunokunxulunyaniswa neemeko zokusingqongileyo eso sityalo sichatshazelwe kuso, njengamanzi amaminerali, ukukhanya kwelanga, inqanaba lophuhliso kunyeisondlo.

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Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Isifo sesibindi, ukuphazamiseka okuqhelekileyo okubangelwahepatitis yentsholongwane, utywala, iikhemikhali ezinetyhefu yesibindi, imikhwa engafanelekanga yokutya kunye nongcoliseko lokusingqongileyo, yinkxalabo yehlabathi jikelele (Papay et al., 2009). Nangona kunjalo, unyango lwezamayeza lwesi sifo ludla ngokuba nzima ukulusebenzisa kwaye lunesiphumo esithintelweyo. Chinese Traditionalamayeza esintu, ephantsi kwemimiselo emininzi esetyenziselwa ukunyanga izifo zesibindi, isasetyenziswa ngokubanzi ngamaTshayina (Zhao et al., 2014).Artemisia capillarisThunb.,I-Asteraceae, ngokutsho kweBencao Gangmu, iirekhodi ezidumileyo zeMithi yaseShayina yeNdabuko, isetyenziswe ngokubanzi njengeyeza lokucima ukushisa, ukukhuthaza.i-diuresiskwaye isuse i-jaundice kwaye isetyenziswe njengencasa kwiziselo, imifuno, kunye neepastries ngenxa yevumba layo elithile.A. capillarisiye yagqalwa njengodidi lwamayeza esintu nokutya kwamaTshayina ngabantu abaninzi. Ke ngoko, kuye kwakho iinzame ezinkulu zokuphuhlisa amayeza esintu aluncedo, anjengalaA. capillaris, kunyango lwesifo sesibindi.

Kwiminyaka yakutshanje, amayeza eengcambu afumene ingqalelo ngakumbi kunye nokuthandwa kunyango lwesifo sesibindi ngenxa yokhuseleko kunye nokusebenza kakuhle (Ding et al., 2012).A. capillarisibonakaliswe ukuba inomsebenzi olungileyo we-hepatoprotective esekwe kwiindlela zanamhlanje ze-pharmacological (Han et al., 2006). Ikwayinto ebalulekileyo yezamayeza eTshayina kwaye iyinto eyaziwayo yokuchasa ukudumba (Cha et al., 2009a),choleretic(UYoon noKim, ngo-2011kunye ne-anti-tumor (Feng et al., 2013)iyeza lesintu.

PhytochemicalUphononongo luveze inani leoyile eziguquguqukayo ezibalulekileyo,iicoumarins, kwayei-flavonol glycosideskwakunye neqela labantu abangaziwayoiiaglyconesukusukaA. capillaris(Komiya et al., 1976,Yamahara et al., 1989). Ioli ebalulekileyo yeA. capillaris(AEO) yenye yeekhompawundi eziphambili ze-pharmacological esebenzayo kwaye inikezela ukuchasana nokudumba (Cha et al., 2009a) kunye neempawu ezichasene ne-apoptotic (Cha et al., 2009b). Nangona kunjalo, njengoko i-AEO yenye yeekhompawundi eziphambili zeA. capillaris, imisebenzi enokubakho ye-hepatoprotective yamacandelo amakhulu ukusukaA. capillariskufuneka iphononongwe.

Kule sifundo, umphumo wokukhusela we-AEO kwicarbon tetrachloride(CCl4)-eyenziwehepatotoxicityyavavanywa ngeendlela ze-biochemical, ezifana ne-hepaticukunciphisa i-glutathione(GSH),malondialdehyde(MDA) amanqanaba,i-superoxide dismutase(SOD), kunyeglutathione peroxidase(GSH-Px) umsebenzi, ngokunjalo nemisebenzi yeaspartate aminotransferase(AST) kunyei-alanine aminotransferase(ALT) kwiserum. Ubungakanani bokulimala kwesibindi okubangelwa yi-CCl4 kwakhona kwahlalutywa ngokuqwalaselwa kwe-histopathological, ehamba kunye nohlalutyo lwe-phytochemical yi-GC-MS ukuchonga amacandelo e-AEO.